human aβ40 elisa kit  (Thermo Fisher)

Journal: The Journal of Prevention of Alzheimer's Disease

Article Title: LMTK2 and CRB1 are two novel risk genes for Alzheimer's disease in Han Chinese

doi: 10.1016/j.tjpad.2025.100087

Figure Lengend Snippet: Effects of the rare variants R234P or S974G of LMTK2 on Tau aggregation, Aβ generation, and apoptosis in vitro . (A) Western blot analysis of phosphorylation levels of Tau and total Tau after Flag-LMTK2 and HA-Tau plasmids were co-transfected into HEK293 cells for 48 h. (B) Quantification of relative protein levels in A ( n = 6). (C) Representative images of K18-induced Tau aggregation after HEK293-Tau RD cells were transfected with LMTK2. After LMTK2 plasmid transfection for 24 h, K18 (1µg/µL) was added, induced for 48 h, and photographed by a fluorescence microscope. Scale bar: 20 µm (D) Quantification of cell with inclusions in C ( n = 6). (E) Western blot analysis of the effect of LMTK2 WT and variants on PP1C-GSK3β signaling pathway. (F) Quantification of relative protein levels in E ( n = 3). (G) ELISA detection of secreted Aβ after LMTK2 WT and variants were transfected into SH-SHY5Y-APPSwe cells ( n = 3). (H) Western blot analysis of BAX and Bcl2 expression after LMTK2 WT and variants were transfected into SH-SHY5Y-APPSwe cells. (I) Quantification of relative BAX and Bcl2 expression ( n = 6). Western blot data are representative of three independent experiments. Data are mean ± SEM, *, * and *** represent p < 0.05, p < 0.01, and p < 0.001, respectively, and ns represents no statistical significance (Student's t -test).

Article Snippet: The supernatants were collected, and the levels of Aβ40 and Aβ42 were determined in accordance with the operating instructions of the human Aβ40 ELISA kit (Elabscience, #E-EL-H0542) and human Aβ42 ELISA kit (Elabscience, #E-EL-H0543), respectively.

Techniques: In Vitro, Western Blot, Phospho-proteomics, Transfection, Plasmid Preparation, Fluorescence, Microscopy, Enzyme-linked Immunosorbent Assay, Expressing

Journal: The Journal of Prevention of Alzheimer's Disease

Article Title: LMTK2 and CRB1 are two novel risk genes for Alzheimer's disease in Han Chinese

doi: 10.1016/j.tjpad.2025.100087

Figure Lengend Snippet: Effect of rare variants in CRB1 on Aβ generation. (A) WB detection of APP and related secretory enzymes expression after high expression of different CRB1 plasmids in SH-SY5Y-APPSwe cells. (B) Quantification of related APP expression. (C-E) ELISA detection of the concentration of Aβ42 (C), Aβ40 (D), and the Aβ42/Aβ40 ratio (E) in the culture medium after the overexpression of different CRB1 plasmids in SH-SY5Y-APPSwe cells. Western blot data are representative of three independent experiments. Data are mean ± SEM, * and ** represent p < 0.05 and p < 0.01, respectively, and ns represents no statistical significance (Student's t -test).

Article Snippet: The supernatants were collected, and the levels of Aβ40 and Aβ42 were determined in accordance with the operating instructions of the human Aβ40 ELISA kit (Elabscience, #E-EL-H0542) and human Aβ42 ELISA kit (Elabscience, #E-EL-H0543), respectively.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay, Over Expression, Western Blot